Txoj Kev Tshiab Mus Rau Qhov Kev Ntsuas Kab Mob Khaus Viv-19 nrawm dua Paved hauv kub

Kev sib kis ceev ceev ntawm COVID-19, ib tus kab mob tshwm sim los ntawm tus kab mob SARS-CoV-2, tau tsim muaj teeb meem kev noj qab haus huv rau pej xeem thoob ntiaj teb. Kev tshawb nrhiav COVID-19 thaum ntxov thiab kev cais tawm yog qhov tseem ceeb rau kev tswj hwm kev kis tus kabmob thiab tiv thaiv cov neeg tsis muaj zog. Tus qauv tam sim no rau kev kuaj mob KEVID-19 yog thim rov qab transcriptase-polymerase saw cov tshuaj tiv thaiv (RT-PCR), cov txheej txheem hauv cov noob kab mob tau kuaj pom tom qab lawv tau dhau ntau lub voj voog ntawm kev nthuav dav. Txawm li cas los xij, cov txheej txheem no siv sijhawm ntev, tsim kev sim rov qab los ntawm cov chaw kuaj mob thiab ua rau ncua kev kuaj mob.      

Hauv kev tshawb fawb tsis ntev los no tau tshaj tawm hauv Biosensors thiab Bioelectronics, cov kws tshawb fawb los ntawm Kaus Lim Kauslim thiab Tuam Tshoj tau qhia txog qhov tshiab nanotechnology-based platform uas tuaj yeem txo lub sijhawm xav tau rau kev kuaj mob COVID-19. Lawv qhov chaw ua kom zoo nkauj Raman tawg (SERS)-PCR nrhiav pom platform-npaj siv cov nanoparticles kub (AuNPs) hauv cov kab noj hniav ntawm Au 'nanodimple' substrates (AuNDSs) - tuaj yeem ntes cov kab mob kis tau tom qab tsuas yog 8 lub voj voog ntawm kev nthuav dav. Qhov ntawd yog yuav luag ib feem peb ntawm tus lej xav tau nrog cov pa RT-PCR.

"Txoj Cai RT-PCR yog raws li kev tshawb pom ntawm cov teeb liab fluorescence, yog li 3-4 teev yuav tsum tau kuaj xyuas SARS-CoV-2. Qhov ceev no tsis txaus los txiav txim siab seb COVID-19 kis tau sai npaum li cas. Peb xav nrhiav txoj hauv kev los txiav lub sijhawm no tsawg kawg ib nrab, "hais tias Prof. Jaebum Choo, piav qhia txog kev txhawb siab tom qab kev kawm. Hmoov zoo, cov lus teb tsis dhau deb. Nyob rau hauv ib txoj kev tshawb fawb yav dhau los luam tawm xyoo 2021, Prof. Choo pab neeg tau tsim ib qho tshiab nrhiav kom tau platform uas muaj siab SERS cov cim qhia tau tsim los ntawm AuNPs sib npaug ntawm cov kab noj hniav ntawm AuNDSs los ntawm cov txheej txheem hu ua DNA hybridization. Raws li qhov kev tshawb pom dhau los no, Prof. Choo thiab nws pab neeg tau tsim kho tshiab SERS-PCR platform rau kev kuaj mob COVID-19.

Qhov kev ntsuam xyuas SERS-PCR tshiab tau tsim siv SERS cov cim los kuaj xyuas "bridge DNA" - cov DNA me me uas maj mam ua rau hauv qhov pom ntawm cov hom kab mob kis. Yog li ntawd, nyob rau hauv cov qauv ntawm cov neeg mob zoo rau COVID-19, qhov concentration ntawm choj DNA (thiab yog li ntawd lub teeb liab SERS) tsis tu ncua nrog kev nce qib PCR. Hauv qhov sib piv, thaum SARS-CoV-2 tsis tuaj, SERS teeb liab tseem tsis hloov.

Pab neeg no tau sim qhov ua tau zoo ntawm lawv cov kab ke siv ob lub hom phiaj lub hom phiaj ntawm SARS-CoV-2, uas yog, lub hnab ntawv protein (E) thiab RNA-dependent RNA polymerase (RdRp) noob ntawm SARS-CoV-2. Thaum 25 lub voj voog xav tau rau RT-PCR-raws li kev tshawb nrhiav, AuNDS-based SERS-PCR platform yuav tsum tsuas yog 8 lub voj voog, txiav txim siab txo lub sijhawm ntsuas. "Txawm hais tias peb cov txiaj ntsig yog qhov ua ntej, lawv muab cov ntaub ntawv pov thawj tseem ceeb rau kev siv tau ntawm SERS-PCR raws li cov txheej txheem kuaj mob. Peb cov txheej txheem AuNDS-based SERS-PCR yog ib qho kev cog lus tshiab molecular diagnostic platform uas tuaj yeem txo lub sij hawm xav tau rau kev tshawb nrhiav noob piv rau cov qauv RT-PCR cov tswv yim. Cov qauv no tuaj yeem nthuav dav ntxiv los ntawm kev sib koom ua tus qauv tsis siv neeg los tsim cov txheej txheem tshuaj ntsuam xyuas molecular tom ntej," piav qhia Prof. Choo.

Tseeb tiag, SERS-PCR tuaj yeem yog lub cuab yeej tseem ceeb hauv peb lub arsenal tiv thaiv COVID-19 kis thoob qhov txhia chaw. Nws kuj tseem tuaj yeem tsim kev hloov pauv hauv thaj chaw ntawm kev tshuaj ntsuam xyuas molecular, hloov pauv peb li cas thiaj kuaj tau cov kab mob sib kis thiab daws cov kab mob sib kis yav tom ntej.

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